Direct Impact of Pomegranate Juice and Peel Extracts on Prostate Cancer Progression - Inhibition of Proliferation, Migration, and Colony Formation in Prostate Cancer Cell Lines
AUTHORS
S. Philp,The University of Sydney, Australia
ABSTRACT
Prostate cancer, which affects millions of men worldwide, is a prevalent form of cancer. The risk factors associated with it include age, genetics, and diet. Despite advances in cancer research, the quest for effective treatments and preventive measures against this disease remains an ongoing challenge. Pomegranate, a fruit known for its antioxidant-rich juice and bioactive peel extracts, has been identified as a prospective therapeutic intervention for various health ailments, including cancer. The current study looked at the direct effects of the juice of pomegranates and peel extract on the advancement of prostate cancer. Its ability to suppress the colony formation, motility, and proliferation of prostate cancer cell lines as cancer cells was assessed. The decline in cell survival after an injection of PP extract was measured using the Mean Transit Time (MTT) test to do this. Prostate cancer cells' ability to form colonies and to be inhibited from migrating was also evaluated using the clonogenic assay and wound healing experiment conducted with the help of pomegranate extracts. The results revealed promising inhibitory effects of pomegranate extracts on these key cancer hallmarks, suggesting their potential as complementary therapeutic agents for prostate cancer.
KEYWORDS
Natural extracts, Anti-cancer, Antioxidants, Pomegranate, Cytotoxicity
REFERENCES
[1] J. E. M. Ferlay, F. Lam, M. Colombet, L. Mery, M. Pineros, A. Znaor, and I. Soerjomataram, “Global cancer observatory: Cancer today,” Lyon, France: International Agency for Research on Cancer, (2023), Available from: https://gco.iarc.fr/today
[2] F. Bray, J. Ferlay, I. Soerjomataram, R. L. Siegel, L. A. Torre, and A. Jemal, “Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries,” CA: A Cancer Journal for Clinicians, vol.68, no.6, pp.394-424, (2018)
[3] J. H. Hayes and M. J. Barry, “Screening for prostate cancer with the prostate-specific antigen test: A review of current evidence,” Jama, vol.311, no.11, pp.1143-1149, (2014)
[4] M. Friedlander, Y. Kapulnik, and H. Koltai, “Plant-derived substances with anti-cancer activity: From folklore to practice,” Frontiers in Plant Science, vol.6, pp.799, (2015)
[5] R L. Riegel, K. D. Miller, and A. Jemal, “Cancer statistics,” CA Cancer J Clin, vol.67, no.1, pp.7-30, (2017)
[6] S. Nobili, D Lippi, E. Witort, M. Donnini, L. Bausi, E. Mini, and S. Capaccioli, “Natural compounds for cancer treatment and prevention,” Pharmacological Research, vol.59, no.6, pp.365-378, (2009)
[7] J. Boik, “Natural compounds in cancer therapy, (2001)
[8] F. Majolo, L. K. D. O. B. Delwing, D. J. Marmitt, I. C. Bustamante-Filho, and M. I. Goettert, “Medicinal plants and bioactive natural compounds for cancer treatment: Important advances for drug discovery,” Phytochemistry Letters, vol.31, pp.196-207, (2019)
[9] O. M. Prakash, A. Kumar, and P. Kumar, “Anti-cancer potential of plants and natural products,” Am J Pharmacol Sci, vol.1, no.6, pp.104-115, (2013)
[10] L. Pan, H. B. Chai, and A. D. Kinghorn, “Discovery of new anti-cancer agents from higher plants,” Frontiers in Bioscience (Scholar edition), vol.4, pp.142, (2012)
[11] T. Koltai, S. J. Reshkin, F. Baltazar, and L. Fliegel, “Prostate cancer metabolism: From biochemistry to therapeutics,” Academic Press, (2021)
[12] M. Viladomiu, R. Hontecillas, P. Lu, and J. Bassaganya-Riera, “Preventive and prophylactic mechanisms of action of pomegranate bioactive constituents,” Evidence-Based Complementary and Alternative Medicine, (2013)
[13] M. M. A. N. Ranjha, B. Shafique, L. Wang, S. Irfan, M. N. Safdar, M. A. Murtaza, and H. R. Nadeem, “A comprehensive review on phytochemistry, bioactivity and medicinal value of bioactive compounds of pomegranate (Punica granatum),” Advances in Traditional Medicine, pp.1-21, (2021)
[14] C. V. S. Prakash and I. Prakash, “Bioactive chemical constituents from pomegranate (Punica granatum) juice, seed, and peel-a review," Int J Res Chem Environ, vol.1, no.1, pp.1-18, (2011)
[15] M. M. G. Karasawa and C. Mohan, “Fruits as prospective reserves of bioactive compounds: A review,” Natural products and bioprospecting, vol.8, pp.335-346, (2018)
[16] S. Akhtar, T. Ismail, D. Fraternale, and P. Sestili, “Pomegranate peel and peel extracts: Chemistry and food features,” Food Chemistry, vol.174, pp.417-425, (2015)
[17] M. López-Lázaro, “Two preclinical tests to evaluate anti-cancer activity and to help validate drug candidates for clinical trials,” Oncoscience, vol.2, no.2, pp.91, (2015)
[18] V. M. Adhami, N. Khan, and H. Mukhtar, “Prevention of cancer with pomegranate and pomegranate anthocyanins,” Berries and Cancer Prevention, pp.209-226, (2011)
[19] A. Bishayee and A. S. Darvesh, “Pomegranate-derived constituents as inducers of cell death: Implications in cancer prevention and therapy,” Natural compounds as inducers of cell death, vol.1, pp.33-47, (2012)
[20] D. N. Syed, F. Afaq, and H. Mukhtar, “Pomegranate derived products for cancer chemoprevention,” In Seminars in Cancer Biology, Academic Press, vol.17, no.5, pp.377-385, (2007)
[21] M. G. Miguel, M. A. Neves, and M. D. Antunes, “Pomegranate (Punica granatum L.): A medicinal plant with myriad biological properties-A short review,” Journal of Medicinal Plants Research, vol.4, no.25, pp.2836-2847, (2010)
[22] N. Panth, B. Manandhar, and K. R. Paudel, “Anti-cancer activity of Punica granatum (pomegranate): A review. Phytotherapy Research, vol.31, no.4, pp.568-578, (2017)
[23] K. Seidi, R. Jahanban-Esfahlan, M. Abasi, and M. M. Abbasi, “Anti tumoral properties of Punica granatum (Pomegranate) seed extract in different human cancer cells,” Asian Pacific Journal of Cancer Prevention, vol.17, no.3, pp.1119-1122, (2016)
[24] F. M. Chaves, I. C. B. Pavan, L. G. S. da Silva, L. B. de Freitas, M. A. Rostagno, A. E. C. Antunes, and F. M. Simabuco, “Pomegranate juice and peel extracts can inhibit proliferation, migration and colony formation of prostate cancer cell lines and modulate the Akt/mTOR/S6K signaling pathway, Plant foods for human nutrition, vol.75, pp.54-62, (2020)
[25] Y. Deng, Y. Li, F. Yang, A. Zeng, S. Yang, Y. Luo, and W. Yin, “The extract from Punica granatum (pomegranate) peel induces apoptosis and impairs metastasis in prostate cancer cells,” Biomedicine & pharmacotherapy, vol.93, pp.976-984, (2017)
[26] L. Wang, A. Alcon, H. Yuan, J. Ho, Q. J. Li, and M. Martins-Green, “Cellular and molecular mechanisms of pomegranate juice-induced anti-metastatic effect on prostate cancer cells,” Integrative Biology, vol.3, no.7, pp.742-754, (2011)
[27] O. D. Keta, M. Deljanin, V. Petkovic, G. Zdunic, T. Jankovic, J. Zivkovic, and K. Savikin, “Pomegranate (Punica granatum L.) peel extract: Potential cytotoxic agent against different cancer cell lines,” Records of Natural Products, vol.14, no.5, pp.326-339, (2020)