Rapid Identification of Cancerous Cell Lines and Evaluation of Cytotoxic Effects on Cancer Cells with VUV-PIMS
AUTHORS
Renjie Song,Otolaryngology Department, Anhui University of Chinese Medicine, 103 Meishan Road, Hefei 230000, China
Feihu Wu,Otolaryngology Department, Anhui University of Chinese Medicine, 103 Meishan Road, Hefei 230000, China
Gang Liu,Otolaryngology Department, Anhui University of Chinese Medicine, 103 Meishan Road, Hefei 230000, China
Ling Zhu,Otolaryngology department, Anhui University of Chinese Medicine, 103 Meishan Road, Hefei 230000, China
Danhua Zhu,Otolaryngology department, Anhui University of Chinese Medicine, 103 Meishan Road, Hefei 230000, China
Zhen Li,State Environmental Protection Engineering Center for Mercury Pollution and Control, Beijing Advanced Sciences and Innovation Center, Chinese Academy of Sciences, Beijing, 101407, China
ABSTRACT
Distinguishing tumor from normal and exploring candidate drugs for anti-cancer activity serve as two of most main impetuses for development of cancer therapeutics. In this study, a vacuum ultraviolet photoionization mass spectrometer (VUV-PIMS) has been applied for the headspace trace-level characteristic of the volatile organic compounds (VOCs) emitted by in-vitro cultured human cells. To validate VUV-PIMS’ potential for cell or tissue diagnostics by cancer-related VOC biomarkers, four species of cancerous and one non-cancerous cell lines were selected, i.e. HeLa, A549, HepG-2, MGC-803 and 293T, derived from cervical carcinoma, lung cancer, hepatoma, gastric cancer and non-malignant control respectively. Furthermore, to clarify whether VUV-PIMS is a proper analytical tool that allow evaluating candidate drug effects against cancerous cell lines, the VOCs profiling of cell lines exposed to 50nM polyphyllin and vitamin C (Vc) were measured as well. As a result, dozens of VOCs were found to be significantly distinctive released between cancerous and non-cancerous cell lines, which can be used as volatile cancer markers. Additionally, dimethyl sulphide (DMS) were substantially more released form cancerous cells than non-cancerous cells after exposure of polyphyllin. Accordingly, we propose a mechanism by which cysteamine is accumulated in cancerous cells and metabolized to DMS on a large scale when confronting with some sort of cytotoxic effects (autophagy and apoptosis). Therefore, this technique is not only reliable enough to recognize cancerous cell lines, but also a supplementary method to explore how drug-related cytotoxic effects will influence the metabolism of cancer cells.
KEYWORDS
VUV-PIMS, Cytotoxic effects, Cancer cells, Dimethyl sulphide
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